749 - 759 Lau Yee Ling.pmd
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چکیده
Malaria causes high global mortality and morbidity annually. Plasmodium knowlesi has been recognised as the fifth human Plasmodium sp. and its infection is widely distributed in Southeast Asia. Merozoite surface protein-119 (MSP-119) appears as a potential candidate for malaria blood stage vaccine as it could induce protective immunity. In this study, codon optimized P. knowlesi MSP-119 (pkMSP-119) was expressed and purified in yeast Pichia pastoris expression system. The purified recombinant protein was further evaluated using Western blot assay using knowlesi malaria, non-knowlesi human malaria, non-malarial parasitic infections and healthy serum samples (n = 50). The sensitivity of purified pkMSP-119 towards detection of knowlesi infection was as 28.6% (2/7). pkMSP-119 did not react with all nonmalarial parasitic infections and healthy donor sera, yet reacted with some non-knowlesi human malaria sera, therefore lead to a specificity of 86.0% (37/43).
منابع مشابه
Sports Med 2009;
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College of Pharmacy, Catholic University of Daegu, Gyeongsan, Gyeongbuk 712-702, Korea. *E-mail: [email protected] College of Oriental Medicine, Dongguk University, Gyeongbuk 780-714, Korea College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu 702-701, Korea College of Pharmacy, Yeungnam University, Gyeongsan 712-749, Korea Faculty of Food Science...
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